Femtosecond laser treatment enhances DNA transfection efficiency in vivo
نویسندگان
چکیده
منابع مشابه
Femtosecond optical transfection of cells: viability and efficiency.
Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was...
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BACKGROUND In molecular medicine, the manipulation of cells is prerequisite to evaluate genes as therapeutic targets or to transfect cells to develop cell therapeutic strategies. To achieve these purposes it is essential that given transfection techniques are capable of handling high cell numbers in reasonable time spans. To fulfill this demand, an alternative nanoparticle mediated laser transf...
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PURPOSE Comparison of resident surgeon performance efficiencies in femtosecond laser-assisted cataract surgery (FLACS) versus conventional phacoemulsification. PATIENTS AND METHODS A retrospective cohort study was conducted on consecutive patients undergoing phacoemulsification cataract surgery performed by senior ophthalmology residents under the supervision of 1 attending physician during a...
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Femtosecond laser pulses have the unique ability to deposit energy into a microscopic volume in the bulk of a material that is transparent to the laser wavelength without affecting the surface of the material. Here we review the use of this capability to disrupt specifically targeted structures in live cells and animals with the goal of elucidating function and modeling disease states. Particul...
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conclusions eudragit coated nanoparticle of chitosan is an efficient choice for oral delivery of dna to upper part of gi tract. results the mean size and zeta potential of particles were 300 ± 4 nm and 14 ± 0.5 mv, respectively. encapsulation of this system was 89.6 ± 1.2%. dna release from batches was less than 12% at ph = 5.2 and more than 60% at ph = 6.8 with significant difference of p < 0....
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ژورنال
عنوان ژورنال: Journal of Biomedical Science
سال: 2009
ISSN: 1423-0127
DOI: 10.1186/1423-0127-16-36